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How could thin layer chromatography be used to monitor the reaction course?

A. By comparing the position and intensity of reactant and product spots on the TLC plate at different time points.
B. Analyzing the changes in Rf values of reactants and products over time.
C. Quantifying the relative amounts of reactants and products using densitometry.
D. Examining the disappearance of reactant spots and appearance of new product spots on the TLC plate.

Answer :

Final answer:

Thin Layer Chromatography (TLC) is a valuable tool in monitoring reaction progress by analyzing spot changes over time and quantifying reactant and product amounts using densitometry and Rf values. So, the correct options are (b) Analyzing the changes in Rf values of reactants and products over time and (c) Quantifying the relative amounts of reactants and products using densitometry.

Explanation:

Thin Layer Chromatography (TLC) can be used to monitor the progress of a reaction by analyzing the changes in the position and intensity of reactant and product spots on the TLC plate over time. The disappearance of reactant spots and appearance of new product spots can signify the completion of the reaction.

By quantifying the relative amounts of reactants and products using densitometry and calculating the Rf values of reactants and products, chemists can track the reaction course accurately.

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