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Answer :
Final answer:
The exponential phase of PCR is analyzed when quantitating DNA or RNA because this phase reflects a direct proportionality between the PCR product and the initial amount of DNA, which allows accurate calculations of the template quantity.
Explanation:
The exponential phase of the polymerase chain reaction, or PCR, is analyzed to quantitate the amount of DNA or RNA in a sample. This phase is chosen because during this phase, the amount of PCR product doubles during each cycle, thus providing a clear indication of DNA amplification which is directly proportional to the initial quantity of the target sequence. The exponential phase is preferable as it occurs before the reaction components, such as dNTPs or the DNA polymerase, become limiting. Specifically, real-time PCR (also known as quantitative PCR or qPCR) employs fluorescence to monitor the amplification of the DNA during this phase, which can then be used to calculate the quantity of the original template DNA in the sample. This is crucial as the exponential phase reflects the most accurate relationship between the PCR product and the initial quantity of template DNA,
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