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Answer :
Final answer:
To examine urine under microscope, collect a sample, centrifuge it, decant supernatant, resuspend the urine sediment, place a drop on a slide with a cover slip, and examine under microscope.
Explanation:
The technical procedure for obtaining precise and reproducible microscopic examination of urine involves several steps. Firstly, collect a urine sample in a clean, sterile container. Then, centrifuge the sample typically at 1500 RPM for about 5 minutes to concentrate the particles.
After centrifugation, decant the supernatant leaving about 1 mL of urine with the sediment. Resuspend the urine sediment by agitating the tube and place one drop on a glass slide. A cover slip is then put in place.
With a microscope, examine the slide under low power objective to identify crystals and casts, and then use high power objective to identify cells, bacteria, and yeast. Always handle and process samples in a timely manner to ensure the prevention of cell lysis, crystallization, or overgrowth of contaminants.
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