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Answer :
Create a calibration curve in Excel, prepare standard solutions, inject them, and note peak regions to quantify chemicals in a BTEX sample using HPLC-UV. To determine the compound concentrations in the sample, plot peak regions against known amounts to produce a calibration curve.
To quantify each compound in a BTEX sample in water using HPLC-UV and build a calibration curve in Excel, we can follow the steps below:
1. Prepare standard solutions of each BTEX compound in water at different concentrations (e.g., 0.1 ppm, 0.5 ppm, 1 ppm, etc.).
2. Inject each standard solution into the HPLC-UV and record the peak areas for each compound.
3. Plot the peak areas against the known concentrations of each standard solution to create a calibration curve.
4. Use the calibration curve to determine the concentrations of each BTEX compound in the sample by injecting the sample into the HPLC-UV and recording the peak areas.
5. Calculate the concentrations of each compound in the sample using the calibration curve.
To build the calibration curve in Excel, we can use the LINEST function to calculate the slope and intercept of the linear regression line. The slope represents the sensitivity of the detector to the compound, while the intercept represents the background noise. We can then use the equation of the line to calculate the concentrations of each compound in the sample.
Conclusion: To quantify each compound in a BTEX sample in water using HPLC-UV and build a calibration curve in Excel, we need to prepare standard solutions of each compound, inject them into the HPLC-UV, and record the peak areas.
By plotting the peak areas against the known concentrations of each standard solution, we can create a calibration curve and use it to determine the concentrations of each compound in the sample. This process is based on the understanding of HPLC-UV, calibration curves, and linear regression analysis in Excel.
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