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In electrophoresis labs, the size of DNA fragments in the gel being analyzed is quantified in terms of their number of base pairs.

What does this mean? Why are some fragments larger than others?

Answer :

The well-known Agarose gel electrophoresis (AGE) procedure may be used to measure the precise sizes of separated DNA fragments by graphing the log of the molecular weight for each band of a DNA standard versus the distance traveled by each band

Since DNA is negatively charged, it will move toward the positively charged electrode when an electric current is supplied to the gel. The DNA fragments are organized in size order because shorter DNA strands travel through the gel more quickly than longer ones during Agarose gel electrophoresis (AGE) .

During Agarose gel electrophoresis (AGE), typically samples and a DNA marker with known-length fragments are passed through the gel at the same time. You may determine the approximate length of the DNA fragments in the samples by comparing the bands of the DNA samples with those from the DNA marker.

To learn more about Agarose gel electrophoresis (AGE) click here,

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